L-Poly(lactic acid) Production by Microwave Irradiation of Lactic Acid Obtained from Lignocellulosic Wastes

L-polylactic acid (PLA), a semi–crystalline aliphatic polyester, is one of the most manufactured biodegradable plastics worldwide. The objective of the study was to obtain L-polylactic acid (PLA) from lignocellulosic plum biomass. Initially, the biomass was processed via pressurized hot water pretreatment at a temperature of 180 °C for 30 min at 10 MPa for carbohydrate separation. Cellulase and the beta-glucosidase enzymes were then added, and the mixture was fermented with Lacticaseibacillus rhamnosus ATCC 7469. The resulting lactic acid was concentrated and purified after ammonium sulphate and n-butanol extraction. The productivity of L-lactic acid was 2.04 ± 0.18 g/L/h. Then, the PLA was synthesized in two stages. Firstly, lactic acid was subjected to azeotropic dehydration at 140 °C for 24 h in the presence of xylene, using SnCl2 (0.4 wt.%) as a catalyst, resulting in lactide (CPLA). Secondly, microwave-assisted polymerization was carried out at 140 °C for 30 min with 0.4 wt.% SnCl2. The resulting powder was purified with methanol to produce PLA with 92.1% yield. The obtained PLA was confirmed using electrospray ionization mass spectrometry, nuclear magnetic resonance, thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. Overall, the resulting PLA can successfully replace the traditional synthetic polymers used in the packaging industry.


Introduction
The production of bioplastics from renewable materials has captured considerable research interests as an opportunity to mitigate greenhouse gas emissions, which have resulted mainly from the combustion of fossil fuels and plastics. Renewable biomass can be used to produce fuels and chemicals as a substitute for oil resources. To produce bioplastics from lignocellulosic feedstocks, the main components of polysaccharides (cellulose and hemicellulose) must be extracted and subjected to pretreatment and hydrolysis [1]. Lignocelluloses are mainly composed of cellulose (25-55%), hemicellulose (11-50%), and lignin (10-40%). Fermentable sugars are utilized as a substrate in the fermentation process for the production of biopolymers, such as poly(lactic acid) (PLA) [2]. The main components of cellulosic biomass (cellulose and hemicellulose) can be converted into PLA, while cellulose and lignin can be transformed into polyhydroxyalkanoate (PHA). Cellulose is a homopolymer of β-D-glucose, with structural units connected by β-1-4 glycoside bonds and the was also used as a biocatalyst for L-lactide polymerization at 90 • C [20,21]. Metal complexes, alcohols, and other compounds are used as initiators of ring opening polymerization. In general, PLA is synthesized by polycondensation and the ring-opening polymerization of lactic acid, as well as by direct polycondensation at high temperatures, with or without the use of catalysts. The catalysts used are metal complexes (Al, Mg, Zn, Ca, Sn, Fe, Y, Sm, Lu, Ti, and Zn). Some studies also reported the production of PLA from various raw materials by polycondensation of lactic acid through microwave irradiation [22,23]. High molecular weight L-poly(lactic acid) (PLA) was synthetized by the ring-opening polymerization of L-lactide in the presence of 1,12-dodecadiol and ditrimethylolpropane as initiators [24].
The current study aims to investigate (a) the pretreatment of lignocellulosic biomass in order to separate the cellulosic components; (b) the simultaneous saccharification and fermentation of cellulose separated from lignocellulosic waste to produce lactic acid; (c) poly(lactic acid) production from L-lactic acid by microwave irradiation; and (d) the structural characterization of the polymer using electrospray ionization mass spectrometry (ESI-MS), proton nuclear magnetic resonance ( 1 H-RMN), X-ray diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Our previous publication described a method for cellulose separation from apple orchard wastes by supercritical carbon dioxide [25]. In this regard, the present study continues the investigation in order to obtain lactic acid from cellulose separated from plum orchard waste, and, finally, the polycondensation of lactic acid to PLA using a more environmentally friendly method.
The plum orchard biomass contains 38.4 ± 1.2% cellulose, 26.8 ± 1.0% hemicelluloses, and 28.6 ± 0.98% lignin. The pretreatment method was used for the separation of cellulose constituents. The solid fraction resulting after the pretreatment contained cellulose, lignin, and small quantities of hemicelluloses (8.1 ± 0.04%). Hemicelluloses were recovered in the liquid fraction as mixtures of sugars (xylose, glucose, mannose, galactose, and arabinose) and secondary byproducts (5-hydroxymethyl furfural and furfural) due to their hydrophilic character. Biomass has a hydrophilic character due to the large number of hydroxyl groups from cellulose and hemicellulose. After pretreatment, the hydrophobic character was obtained as a result of the elimination of hemicelluloses in the water extract. This property is needed for the subsequent enzymatic hydrolysis process. The solid yield obtained after the pretreatment method was 62.2%. According to the literature, eliminating inhibitory compounds prior to enzymatic hydrolysis and using different lignin blockers could improve the performance of enzymatic hydrolysis [25]. The high cellulose content (48.5 ± 1.6 g/100 g pretreated biomass) obtained from the pretreated biomass is recommended as a substrate for microbial fermentation. A delignification method was applied in order to separate only the cellulose. The solid yield of delignification was 42.5 ± 2.4%. The delignified solid contains 98% cellulose. The pretreated and delignified solids were used for the SSF process.

SSF Process
The pretreated and delignified biomass from the plum orchard was used in the SSF process for lactic acid production. Cellulose can be degraded by a group of enzymes that contain the cellulase complex: endoglucanase, exoglucanase, and β-glucosidase [26]. In this sense, Trichoderma reesei and β-glucosidase were used in this research, and the recommended temperature for hydrolysis was 50 • C at a pH of 5-5.5. The strain Lacticaseibacillus rhamnosus ATCC 7469, used for fermentation, can produce only L(+)-lactic acid at a recommended temperature of 37 • C at a pH of 5-6. In the SSF process, enzymatic hydrolysis and fermentation were carried out simultaneously in the same bioreactor. The cellulose can be hydrolyzed into glucose which can then be fermented to lactic acid. The SSF process was initiated by simultaneously adding enzyme mixtures and microbial inoculum. The lactic acid production during the SSF of the delignified biomass is presented in Figure 1. In order to avoid the formation of enzyme inhibitors, the enzymatic hydrolysis and fermentation occur simultaneously. in order to separate only the cellulose. The solid yield of delignification was 42.5 ± 2.4%. The delignified solid contains 98% cellulose. The pretreated and delignified solids were used for the SSF process.

SSF Process
The pretreated and delignified biomass from the plum orchard was used in the SSF process for lactic acid production. Cellulose can be degraded by a group of enzymes that contain the cellulase complex: endoglucanase, exoglucanase, and β-glucosidase [26]. In this sense, Trichoderma reesei and β-glucosidase were used in this research, and the recommended temperature for hydrolysis was 50 °C at a pH of 5-5.5. The strain Lacticaseibacillus rhamnosus ATCC 7469, used for fermentation, can produce only L(+)-lactic acid at a recommended temperature of 37 °C at a pH of 5-6. In the SSF process, enzymatic hydrolysis and fermentation were carried out simultaneously in the same bioreactor. The cellulose can be hydrolyzed into glucose which can then be fermented to lactic acid. The SSF process was initiated by simultaneously adding enzyme mixtures and microbial inoculum. The lactic acid production during the SSF of the delignified biomass is presented in Figure  1. In order to avoid the formation of enzyme inhibitors, the enzymatic hydrolysis and fermentation occur simultaneously. According to Figure 1, the highest lactic acid was produced (94.0 ± 3.1 g/L) at 37 °C and at a pH of 5.5, corresponding to 0.86 ± 0.04 g lactic acid/g of glucose after 48 h of fermentation. The experiments were carried out at 50 °C to favor the cellulase enzyme activities, demonstrating that lactic acid is produced in a low concentration (below 5.1 ± 0.1 g/L) in both cases of the tested pH values. Karnaouri et al. reached the same conclusion regarding D-lactic production by the Lactobacillus delbrueckii species [17]. The largest amount of L-LA was produced in the first 48 h of the fermentation process. At the end of the process, the pH of the broth was 4.75. The lactic acid recovery was 90.2%. After 48 h, the residual reducing sugar was 0.73 ± 0.02%.
According to a study conducted by Chen et al., a high cellulase loading in the SSF process substantially improved the enzymatic hydrolysis [27]. In total, 25 FPU/g was used in this study to enhance the enzymatic hydrolysis of celluloses and further fermentation to L-LA acid. Bahry et al. reported that lactic acid production from carob pod waste using L. rhamnosus encapsulated in alginate beads generated 22 g/L, a yield of 76.9%, and a productivity of 1.22 g/L/h from 65 g/L total sugars [28]. Besides lactic acid, impurities of acetic, citric, formic, propionic, and succinic acid were also identified. According to Figure 1, the highest lactic acid was produced (94.0 ± 3.1 g/L) at 37 • C and at a pH of 5.5, corresponding to 0.86 ± 0.04 g lactic acid/g of glucose after 48 h of fermentation. The experiments were carried out at 50 • C to favor the cellulase enzyme activities, demonstrating that lactic acid is produced in a low concentration (below 5.1 ± 0.1 g/L) in both cases of the tested pH values. Karnaouri et al. reached the same conclusion regarding D-lactic production by the Lactobacillus delbrueckii species [17]. The largest amount of L-LA was produced in the first 48 h of the fermentation process. At the end of the process, the pH of the broth was 4.75. The lactic acid recovery was 90.2%. After 48 h, the residual reducing sugar was 0.73 ± 0.02%.
According to a study conducted by Chen et al., a high cellulase loading in the SSF process substantially improved the enzymatic hydrolysis [27]. In total, 25 FPU/g was used in this study to enhance the enzymatic hydrolysis of celluloses and further fermentation to L-LA acid. Bahry et al. reported that lactic acid production from carob pod waste using L. rhamnosus encapsulated in alginate beads generated 22 g/L, a yield of 76.9%, and a productivity of 1.22 g/L/h from 65 g/L total sugars [28]. Besides lactic acid, impurities of acetic, citric, formic, propionic, and succinic acid were also identified.
In the SSF of the pretreated biomass, 53.0 ± 1.8 g/L of potential glucose was introduced in a bioreactor. The presence of a small hemicellulose quantity in the pretreated biomass can be hydrolyzed first and act as an inhibitor of enzymatic hydrolysis. After 24 h of fermentation, 45.95 ± 2.1 g/L of lactic acid was produced at an efficiency of 73.3% (taking into account hemicelluloses). After 44 h of fermentation, glucose was transformed into lactic acid. A pH of 5.5 and a temperature of 50 • C were adequate for producing high lactic acid. By comparing the delignified and pretreated biomass substrates used in the SSF process, it can be inferred that the pretreated substrate can produce a substantial quantity of lactic acid, without requiring a prior delignification method. The SSF process for lactic acid has the benefit of producing lactic acid from pretreated biomass compared to the SSF process for ethanol production, where a delignification method has substantially improved the ethanol yield [24].
The mass balance for L-LA production from lignocellulosic biomass is presented in Figure 2. From 100 ± 3.2 g of lignocellulosic biomass, 65 ± 2.6 g of the solid phase was separated after pressurized hot water pretreatment. The SSF process was finalized by producing 29.2 ± 1.1 g of L-LA. The resulting L-LA was further used to produce L-poly(lactic acid) (PLA).
In the SSF of the pretreated biomass, 53.0 ± 1.8 g/L of potential glucose was introduced in a bioreactor. The presence of a small hemicellulose quantity in the pretreated biomass can be hydrolyzed first and act as an inhibitor of enzymatic hydrolysis. After 24 hours of fermentation, 45.95 ± 2.1 g/L of lactic acid was produced at an efficiency of 73.3% (taking into account hemicelluloses). After 44 h of fermentation, glucose was transformed into lactic acid. A pH of 5.5 and a temperature of 50 °C were adequate for producing high lactic acid. By comparing the delignified and pretreated biomass substrates used in the SSF process, it can be inferred that the pretreated substrate can produce a substantial quantity of lactic acid, without requiring a prior delignification method. The SSF process for lactic acid has the benefit of producing lactic acid from pretreated biomass compared to the SSF process for ethanol production, where a delignification method has substantially improved the ethanol yield [24].
The mass balance for L-LA production from lignocellulosic biomass is presented in Figure 2. From 100 ± 3.2 g of lignocellulosic biomass, 65 ± 2.6 g of the solid phase was separated after pressurized hot water pretreatment. The SSF process was finalized by producing 29.2 ± 1.1 g of L-LA. The resulting L-LA was further used to produce L-poly(lactic acid) (PLA). In Table 2, the lactic acid concentrations obtained in the current study are compared with those reported in the literature. Table 2. Lactic acid concentration obtained in the current study and the results reported in the literature.

Raw Material
Pretreatment Used Microorganism L-Lactic Acid Reference C (g/L) Y (g/g) P (g/L/h) Forest and marginal lands lignocellulosic  In Table 2, the lactic acid concentrations obtained in the current study are compared with those reported in the literature. Table 2. Lactic acid concentration obtained in the current study and the results reported in the literature.

Raw Material
Pretreatment Used Microorganism L-Lactic Acid Reference C (g/L) Y (g/g) P (g/L/h)

Microwave Assisted Polymerization of Lactic Acid to PLA
The polymerization of lactic acid into PLA takes place in two steps: (a) azeotropic dehydration for 24 h and (b) microwave-assisted polymerization at 140 • C and for 30 min to obtain a high molecular mass of PLA. Water removal was carried out by azeotropic distillation using oxylene as solvent (i.e., initiator of the reaction) and SnCl 2 as catalyst (0.4 wt.%). Considering that obtaining high molecular-weight PLA by direct polycondensation is difficult [32], the elimination of water was performed using a Dean-Stark trap and molecular sieves before microwave irradiation was applied. The obtained PLA (PLA 1) was purified by precipitation in cold methanol with a yield of 92.1%.

ESI (+)-HRMS Spectra
The chemical structure of linear PLA is presented in Figure 3. Figure 4 shows an ESI (+)-HRMS spectrum of sample PLA 2. The most abundant form obtained in the polymerization reaction is PLA, with a polymerization degree of n = 4-29 [M n +H 2 O+Na] + . In the mass spectrum, K + adducts were also detected. CPLA [M n +Na] + adducts were also found in low quantities as a byproduct. The measured m/z for [M 12 +H 2 O+Na] + is 905.2519, and the calculated m/z is 905.2533, yielding a difference in ppm of 1.55, which confirms the structure of the proposed poly(lactic acid). M12 indicates the polymer with a polymerization degree of n = 12. In the case of PLA standard, in the ESI-MS spectrum ( Figure 5), a 615 m/z fragment appears, which can be attributed to the [M 16 +CH 3 OH+2Na] 2+ adduct [33]. The presence of alkali metals originates from the ambient contaminants. distillation using oxylene as solvent (i.e., initiator of the reaction) and SnCl2 as cata wt.%). Considering that obtaining high molecular-weight PLA by direct polyco tion is difficult [32], the elimination of water was performed using a Dean-Stark t molecular sieves before microwave irradiation was applied. The obtained PLA was purified by precipitation in cold methanol with a yield of 92.1%.

ESI (+)-HRMS Spectra
The chemical structure of linear PLA is presented in Figure 3. Figure 4 show (+)-HRMS spectrum of sample PLA 2. The most abundant form obtained in the p ization reaction is PLA, with a polymerization degree of n = 4-29 [Mn+H2O+Na] mass spectrum, K + adducts were also detected. CPLA [Mn+Na] + adducts were als in low quantities as a byproduct. The measured m/z for [M12+H2O+Na] + is 905.2 the calculated m/z is 905.2533, yielding a difference in ppm of 1.55, which conf structure of the proposed poly(lactic acid). M12 indicates the polymer with a poly tion degree of n = 12. In the case of PLA standard, in the ESI-MS spectrum (Fig  615 m/z fragment appears, which can be attributed to the [M16+CH3OH+2Na] 2+ add The presence of alkali metals originates from the ambient contaminants.   distillation using oxylene as solvent (i.e., initiator of the reaction) and SnCl2 as catal wt.%). Considering that obtaining high molecular-weight PLA by direct polycon tion is difficult [32], the elimination of water was performed using a Dean-Stark tr molecular sieves before microwave irradiation was applied. The obtained PLA (P was purified by precipitation in cold methanol with a yield of 92.1%.

ESI (+)-HRMS Spectra
The chemical structure of linear PLA is presented in Figure 3. Figure 4 shows (+)-HRMS spectrum of sample PLA 2. The most abundant form obtained in the po ization reaction is PLA, with a polymerization degree of n = 4-29 [Mn+H2O+Na] + . mass spectrum, K + adducts were also detected. CPLA [Mn+Na] + adducts were also in low quantities as a byproduct. The measured m/z for [M12+H2O+Na] + is 905.251 the calculated m/z is 905.2533, yielding a difference in ppm of 1.55, which confir structure of the proposed poly(lactic acid). M12 indicates the polymer with a polym tion degree of n = 12. In the case of PLA standard, in the ESI-MS spectrum (Figu 615 m/z fragment appears, which can be attributed to the [M16+CH3OH+2Na] 2+ addu The presence of alkali metals originates from the ambient contaminants.   In the PLA 1 sample, ESI-HRMS spectra fragments which occur in the case of linear polylactic acid PLA 2 can be observed. In addition, fragments such as m/z 615 are present in the mass spectra. These can be attributed to the adduct with solvent and sodium [M16+CH3OH+2Na] 2+ . The fragments corresponding to the CPLA molecule are present in the MS spectrum, in this case with higher intensity compared to the PLA 2 sample. The ESI-MS spectrum of PLA 1 is presented in Figure 6. The PLA obtained was confirmed by 1 H-RMN spectroscopy. According to the structure presented in Figure 1, PLA 1 presents two groups, -OH and -COOH, at the end of the In the PLA 1 sample, ESI-HRMS spectra fragments which occur in the case of linear polylactic acid PLA 2 can be observed. In addition, fragments such as m/z 615 are present in the mass spectra. These can be attributed to the adduct with solvent and sodium [M 16 +CH 3 OH+2Na] 2+ . The fragments corresponding to the CPLA molecule are present in the MS spectrum, in this case with higher intensity compared to the PLA 2 sample. The ESI-MS spectrum of PLA 1 is presented in Figure 6. In the PLA 1 sample, ESI-HRMS spectra fragments which occur in the case of linear polylactic acid PLA 2 can be observed. In addition, fragments such as m/z 615 are present in the mass spectra. These can be attributed to the adduct with solvent and sodium [M16+CH3OH+2Na] 2+ . The fragments corresponding to the CPLA molecule are present in the MS spectrum, in this case with higher intensity compared to the PLA 2 sample. The ESI-MS spectrum of PLA 1 is presented in Figure 6.

Proton Nuclear Magnetic Resonance ( 1 H-RMN)
The PLA obtained was confirmed by 1 H-RMN spectroscopy. According to the structure presented in Figure 1, PLA 1 presents two groups, -OH and -COOH, at the end of the structure. The 1 H-RMN spectra of the PLA, before and after purification, and CPLA are The PLA obtained was confirmed by 1 H-RMN spectroscopy. According to the structure presented in Figure 1, PLA 1 presents two groups, -OH and -COOH, at the end of the structure. The 1 H-RMN spectra of the PLA, before and after purification, and CPLA are presented in Figure 7a-c. The resonance of the signal present at 1.56-1.58 ppm (d, 3H, and CH 3 ) was attributed to the methyl protons. The signal present at 5.12-5.18 ppm was attributed to the methine protons (-CH) (q, 1H, and CH). The PLA was formed by CPLA polymerization, as confirmed by the 1 H-RMN of CPLA (Figure 7c). The spectrum presents two signals: 5.13-5.22 ppm, attributed to -CH, and 1.53-1.58 ppm, attributed to the CH 3 groups from the lactide structure. The confirmed structure of PLA is in accordance with the study of Suganuma et al., which confirms the NMR analysis of poly(lactic acid) via a statistical model [34]. The 1 H-RMN spectra were used to estimate the molecular weight of the obtained PLA. The molecular weight of PLA 1 was expressed as an average because the polymer contains a mixture of atoms of different lengths. The average molecular weight of the PLA (Mn) was estimated according to Phuphuak et al. [35] and Viamonte-Aristizábal et al. [21]. The terminal units contain a hydroxyl group and carboxylic acid (Figure 8). The 1 H-RMN spectra of the PLA 1 and PLA 2 samples are presented in Figure 9. Signals located at δH = 5.19 and 1.60 ppm were assigned to the methine (CH, c) and methyl (CH3, a) groups in the polymer; meanwhile, the signal located at δH = 4.37 ppm was attributed to the methine end-group (CH, b).

=
• + (1) Signals located at δH = 5.19 and 1.60 ppm were assigned to the methine (CH, c) and methyl (CH3, a) groups in the polymer; meanwhile, the signal located at δH = 4.37 ppm was attributed to the methine end-group (CH, b).

=
• + (1) Signals located at δ H = 5.19 and 1.60 ppm were assigned to the methine (CH, c) and methyl (CH 3 , a) groups in the polymer; meanwhile, the signal located at δ H = 4.37 ppm was attributed to the methine end-group (CH, b). M n = M w reapiting unit · n + (M w end unit ) where M w reapiting unit is the mass of eparative units, M w end unit is the mass of terminal units, c is the intensity of the signal in the proton spectrum of the methine end-group, and b is the intensity of the signal in the proton spectrum of methine from the repeating unit. The n of the PLA structure was calculated from the 1 H-RMN abundance and by dividing the intensity of the signal in the proton spectrum of the methine end-group and the methine proton from the repeating unit peaks. The intensity of the methine proton signal located at 4.37 ppm was significantly lower than the internal methine. The n calculated for PLA 1 was 22.12 and was approximated to 22, and the calculated n for PLA 2 was 14.10 and was approximated to 14. The M n of the PLA calculations was performed as follows: M PLA 1 = (72 · 22) + (72 + 17 + 1) = 1674 g/mol M PLA 2 = (72 · 14) + (72 + 17 + 1) = 1098 g/mol

FTIR Spectrum
The FTIR spectrum for PLA 1 obtained after purification is presented in Figure 10. The chemical structure of PLA was confirmed by the FTIR scan between 4000 and 600 cm −1 . The structure presents the following bands: 1749 cm −1 attributed to the carbonyl stretching (C=O), 1455 cm −1 is the CH 3 asymmetrical scissoring band, 1188 cm −1 attributed to the C-O and C-O-C vibration, 1045 cm −1 is the band of C-CH 3 , and 870 cm −1 is the band of C-COO. Two -C-H bonds were obtained at 2999 cm −1 (asymmetric) and 2948 cm −1 (symmetric). The -OH bond attributed to carboxylic acid occurred at 3500 cm −1 . The -C=O and OH bonds were identified at 1216 cm −1 and 1082 cm −1 . The distinct peak at 1361 cm −1 indicated the semi-crystalline structure of the obtained polymer. The same is the mass of reperative units, is the mass of term units, c is the intensity of the signal in the proton spectrum of the methine end-group b is the intensity of the signal in the proton spectrum of methine from the repeating The n of the PLA structure was calculated from the 1 H-RMN abundance and b viding the intensity of the signal in the proton spectrum of the methine end-group the methine proton from the repeating unit peaks. The intensity of the methine p signal located at 4.37 ppm was significantly lower than the internal methine. The n c lated for PLA 1 was 22.12 and was approximated to 22, and the calculated n for PLA 2 14.10 and was approximated to 14

TGA Analysis
Thermogravimetric analysis (TGA) provides information about the weight loss step. Figure 11 presents the TGA/DTG curves of the PLA samples. The thermal degradation of PLA, before and after purification, and lactide takes place in one stage. PLA purification has a significant effect on the thermal degradation by enhancing thermal stability. Based on the TGA curve, no significant loss of biomass was obtained for PLA 1, while a significant loss of biomass was obtained for the CPLA product. The thermal degradation of PLA begins around 300 • C and ends around 400 • C. The maximum degradation temperature of PLA before purification was 316.45 • C, whereas the temperature of PLA after purification increased to 361.62 • C. The degradation temperature of lactide was 253.2 • C. These higher degradation temperatures for PLA, before and after purification, could be attributed to an increase in PLA molecular weight and to a good thermostability compared with the TGA/DTG of CPLA, which show a low thermostability. The PLA compound has only one degradation stage and it corresponds to the cleavage of the bonds on the polymer with the degradation of lactide, oligomers, acetaldehyde, and carbon monoxide. Some loss of water prior to 200 • C was observed for PLA 2 ( Figure 11). The high stability of PLA 1 results from the presence of hydroxide groups [36]. The TGA for PLA demonstrated that it can be used to replace the synthetic polymer used in packaging. Thermogravimetric analysis (TGA) provides information about the weight loss step. Figure 11 presents the TGA/DTG curves of the PLA samples. The thermal degradation of PLA, before and after purification, and lactide takes place in one stage. PLA purification has a significant effect on the thermal degradation by enhancing thermal stability. Based on the TGA curve, no significant loss of biomass was obtained for PLA 1, while a significant loss of biomass was obtained for the CPLA product. The thermal degradation of PLA begins around 300 °C and ends around 400 °C. The maximum degradation temperature of PLA before purification was 316.45 °C, whereas the temperature of PLA after purification increased to 361.62 °C. The degradation temperature of lactide was 253.2 °C. These higher degradation temperatures for PLA, before and after purification, could be attributed to an increase in PLA molecular weight and to a good thermostability compared with the TGA/DTG of CPLA, which show a low thermostability. The PLA compound has only one degradation stage and it corresponds to the cleavage of the bonds on the polymer with the degradation of lactide, oligomers, acetaldehyde, and carbon monoxide. Some loss of water prior to 200 °C was observed for PLA 2 ( Figure 11). The high stability of PLA 1 results from the presence of hydroxide groups [36]. The TGA for PLA demonstrated that it can be used to replace the synthetic polymer used in packaging.

XRD Analysis
The similar XRD patterns of purified and unpurified PLA are presented in Figure 12. The characteristic diffraction peaks in 2θ angles positioned at 2θ 12.42, 14.76, 16.45, 19.02,

XRD Analysis
The similar XRD patterns of purified and unpurified PLA are presented in Figure 12.  -064-1624). Generally, polymers can comprise crystalline regions randomly mixed with amorphous regions. Moreover, high intensity peaks indicate a polymer with a crystalline structure, while an amorphous structure results in ramps and wider bands [20]. The degree of crystallinity (75% for PLA 1 and 70% for PLA 2), calculated as the ratio between the crystalline regions over the sum of crystalline and amorphous area [37], was considerably higher than in the PLA obtained by the ringopening polymerization of L-lactide using Diutina rugosa lipase [20].  (217), confirm the presence of a pure, crystallized PLA phase of orthorhombic structure (PDF 00-064-1624). Generally, polymers can comprise crystalline regions randomly mixed with amorphous regions. Moreover, high intensity peaks indicate a polymer with a crystalline structure, while an amorphous structure results in ramps and wider bands [20]. The degree of crystallinity (75% for PLA 1 and 70% for PLA 2), calculated as the ratio between the crystalline regions over the sum of crystalline and amorphous area [37], was considerably higher than in the PLA obtained by the ring-opening polymerization of L-lactide using Diutina rugosa lipase [20].

Scanning Electron Microscopy (SEM)
The SEM analysis of the PLA products obtained is presented in Figure 13. Energy dispersive X-ray spectroscopy (EDX) was used to determine the elemental composition of PLA 1 (the final product) and revealed a content of 65.01% C and 34.99% O. The SEM morphology of PLA 1 shows fine particle sizes of a smooth surface with uniform pores. The elemental compositions of CPLA show 58.96% C and 41.04% O. The morphology of CPLA (Figure 13c) shows an instable structure with cracks and large voids. There is a notable difference among the three structures. The surface area of the fiber will grow as the roughness increases [38].

Scanning Electron Microscopy (SEM)
The SEM analysis of the PLA products obtained is presented in Figure 13. Energy dispersive X-ray spectroscopy (EDX) was used to determine the elemental composition of PLA 1 (the final product) and revealed a content of 65.01% C and 34.99% O. The SEM morphology of PLA 1 shows fine particle sizes of a smooth surface with uniform pores. The elemental compositions of CPLA show 58.96% C and 41.04% O. The morphology of CPLA (Figure 13c) shows an instable structure with cracks and large voids. There is a notable difference among the three structures. The surface area of the fiber will grow as the roughness increases [38].  (217), confirm the presence of a pure, crystallized PLA phase of orthorhombic structure (PDF 00-064-1624). Generally, polymers can comprise crystalline regions randomly mixed with amorphous regions. Moreover, high intensity peaks indicate a polymer with a crystalline structure, while an amorphous structure results in ramps and wider bands [20]. The degree of crystallinity (75% for PLA 1 and 70% for PLA 2), calculated as the ratio between the crystalline regions over the sum of crystalline and amorphous area [37], was considerably higher than in the PLA obtained by the ring-opening polymerization of L-lactide using Diutina rugosa lipase [20].

Scanning Electron Microscopy (SEM)
The SEM analysis of the PLA products obtained is presented in Figure 13. Energy dispersive X-ray spectroscopy (EDX) was used to determine the elemental composition of PLA 1 (the final product) and revealed a content of 65.01% C and 34.99% O. The SEM morphology of PLA 1 shows fine particle sizes of a smooth surface with uniform pores. The elemental compositions of CPLA show 58.96% C and 41.04% O. The morphology of CPLA (Figure 13c) shows an instable structure with cracks and large voids. There is a notable difference among the three structures. The surface area of the fiber will grow as the roughness increases [38].

Sample Description
The plum orchard biomass was purchased from the Research Station of the University of Agricultural Sciences "Ion Ionescu de la Brad" in Iasi, Romania. The biomass contained plum tree branches and trunks obtained after orchard pruning. The samples were dried at 105 • C and shredded to a diameter of 0.2 mm.

Pressurized Hot Water Pretreatment of Raw Biomass
Pressurized hot water pretreatment was carried out according to our previous publication method with modifications [24]. The raw material (30 g) and 270 mL of water were introduced in a Parr reactor (Parr Instruments, Moline, IL, USA), equipped with a temperature controller and a 1 L reaction vessel, and heated at a temperature of 180 • C for 30 min at 10 MPa. The solid fraction was separated by filtration and analyzed for cellulose, lignin, and hemicellulose content.

Delignification of Pretreated Biomass
The solid fraction resulting from the pretreatment method was subjected to delignification with sodium chlorite. About 5 g of the pretreated biomass was mixed with 250 mL of acetic acid 10% and 5 g of NaClO 2 . The mixture was heated at 80 • C for 4 h. The solid fraction was separated by filtration and analyzed in regard to the cellulose and residual lignin content.

Simultaneous Saccharification and Fermentation Process SSF to Lactic Acid
The solid fractions that resulted after pretreatment and delignification were introduced in a 1.7 L bioreactor (Lambda Minifor, Lambda Laboratory Instruments, Brno, Czech Republic) equipped with a sensor for dissolved oxygen, pH, and temperature. The solid loading was 10%, and the nutrient (like MRS without glucose) was sterilized at 121 • C for 15 min. CaCO 3 (4%) was added before adjusting the pH. The cellulase from Trichoderma reesei ATCC 26921 and β-glucosidase from almonds were used for the enzymatic hydrolysis of cellulose into glucose. In all the experiments, a 25 FPU/g substrate of enzymes from Trichoderma reesei and 20 U/g of β-glucosidase were used. The inoculum of L. rhamnosus was 10% (v/v). The experiments were conducted at two temperatures: 37 • C and 44 • C for 72 h. Additionally, two pH values were tested: 5.5 and 6.5. During each experiment, 1 mL of sample was taken and boiled for 5 min until the enzymes' deactivation, centrifuged at 5000 rpm for 10 min in order to remove the precipitate, and then used for lactic acid and residual sugar analysis.

Preparation of Stock Culture and Cultivation Conditions
The strain used for the fermentation of cellulose was L. rhamnosus ATCC 7469. A pre-inoculum of L. rhamnosus ATCC 7469 was prepared in sterilized (at 121 • C for 15 min.) broth medium containing 20 g/L glucose, 5 g/L yeast extract, 10 g/L enzymatic digest of casein, 10 g/L meat extract, 5 g/L sodium acetate, 2 g/L diammonium citrate, 2 g/L K 2 HPO 4 , and 0.2 g/L MgSO 4 . The inoculated medium was incubated at 37 • C, in aerobic conditions for 48 h, and stored at 4 • C as a stock culture. Every week, a fresh working culture was prepared from the stock culture in order to maintain the viability of the strain. For the fermentation step, a fresh L. rhamnosus inoculum was prepared from the working culture in 5 mL NaCl at an optical density (OD) of OD600 nm = 4.

Purification of Lactic Acid
The fermentation broth was extracted with a mixture of ammonium sulfate and nbutanol according to the method of Kumar et al. [39]. The fermentation broth was treated with 10 g of ammonium sulfate and then 50 mL of n-butanol was added to the mixture and stirred. The organic phase was separated and evaporated to dryness.

Synthesis of PLA
About 20 g of L-lactic acid obtained by fermentation was introduced in a 250 mL reaction vessel equipped with a Dean-Stark trap. SnCl 2 was used as a catalyst (0.4 wt.%) and 30 mL of xylene was added to the mixtures. The mixtures were refluxed at 140 • C for 24 h under controlled agitation in order to remove the water. The molecular sieves were used as drying agents. After the complete elimination of water, the mixtures were completely distilled. The obtained lactide (CPLA) was purified by recrystallization in ethyl acetate, dried, and heated using microwave irradiation in a microwave reactor (Synthos 3000, Anton Paar, Australia) at 140 • C for 30 min at a maximum pressure of 60 bar with 0.4 wt.% SnCl 2 . The resulting mixture was poured into cold methanol, and the white precipitate (PLA 2) was filtered and dried under reduced pressure. The recrystallization of PLA 2 in methanol led to purified PLA 1 [18].

Chemical Characterization of Raw and Pretreated Biomass
The contents of cellulose, hemicelluloses, and lignin were determined according to Teramoto et al. [40].

Determination of Reducing Sugars
The concentration of reducing sugar was determined by using a Lambda 25 ultraviolet visible spectrometer (Perkin Elmer), according to the Miller method [41]. The DNS reagent was prepared by mixing 40 mL of water with 1 g of 3.5-dinitrisalycilic acid and 2.075 mL of NaOH 50%. The obtained solution was mixed with 30 g of potassium and sodium tartrate, and the final volume of the solution was 100 mL with water. A quantity of 1 mL of hydrolysate solution was mixed with 3 mL of DNS reagent, and the solution was boiled for 5 min. The solution absorbance was measured at 540 nm. The reduced sugars were calculated using Equation (3): Reduced sugars (%) = W S /W 1 (1 × 1000) × 100 (3) where W S is the quantity of sugars determined by reaction with DNS (mg) and W 1 is the initial sample (g). WS is calculated with Equation (4): where C DNS is the concentration of sugars (mg/mL), V is the total volume of hydrolysates (mL), and 0.9 is the conversion factor of the cellulose transformation into glucose.

Determination of Lactic Acid Concentration
The lactic acid produced by the SSF process was determined at a 390 nm wavelength using a Lambda 25 spectrophotometer (Perkin Elmer, Beaconsfield, UK) with 1 cm glass cells, according to Borshchevskaya et al. [42]. Lactic acid (90%) was used as a standard for the calibration curve at a concentration ranging between 0.5 and 10 g/L, by diluting the stock solution with ultrapure water. A volume of 50 µL of cultural liquid was treated with 2 mL of 0.2% FeCl 3 ·6H 2 O solution, and then the absorbance at 390 nm was measured. NMR spectra were recorded at room temperature on a Bruker Advance instrument ( 1 H: 400 MHz) using a CDCl 3 solvent. The NMR software MestReNova was used for the data analysis and processing. The sample (0.3 g) was dried in a vacuum oven at 40 • C and then dissolved in deuterated CDCl 3 (1 mL) before characterization. HRMS spectra were recorded on a Thermo Scientific LTQ Orbitrap XL. The samples were dissolved in anhydrous methanol before analysis.

TGA/DTG Analysis
The thermal decomposition and derivative thermogravimetric (DTG) analysis of PLA and lactide were determined using a TA Instruments SDT O 600 (TA Instruments, New Castle, DE, USA) at temperatures ranging from 30 to 1000 • C at 10 • C per minute under air. A mass of 7.78 ± 0.3 mg of the dried samples was used for testing, and the experiment was conducted under a nitrogen atmosphere. The experiments were repeatable with a standard deviation in the peak temperature values.

FTIR Spectroscopy
The FTIR spectrum of PLA was recorded using a Bruker Vector 22 FT-IR spectrometer (Bruker, Billerica, MA, USA) in the range of 4000-600 cm −1 on 1% KBr pellets in order to identify different functional groups and monitor changes occurring at the functional group level during different conversion processes.

SEM Analysis
The morphology of PLA before and after purification was examined using a scanning electron microscope (SEM VEGAS 3 SBU, Tescan, Brno-Kohoutovice, Czech Republic) with an EDX detector. The samples were deposited on double-sided conductive carbon tape on aluminum stubs and analyzed.

Conclusions
In this study, plum orchard biomass was used for the first time as a raw material for poly(lactic acid) production by the fermentative pathway of carbohydrates obtained by supercritical CO 2 extraction. The solid fraction that resulted after the pretreatment was used for lactic acid fermentation in the SSF process, utilizing Lactocaseibacillus rhamnosus ATCC 7469. The experimental data proved that the microwave irradiation of lactic acid substantially improved the molecular weight of PLA. The study confirmed that plum orchard biomass can be converted into L-poly(lactic acid) via the following steps: pretreatment, simultaneous saccharification and fermentation processes, polycondensation and polymerization under microwave irradiation, and, finally, purification. Thermogravimetric analysis showed a good thermostability of the purified PLA. The structure of the obtained product was confirmed by electrospray ionization mass spectrometry and proton nuclear magnetic resonance. The ESI-MS spectra of PLA 1 and CPLA included Na + and K + adduct ions. The integration of the proposed process into the ecological and sustainable production of valuable products would make a significant contribution to the development of a cleaner chemical industry.